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Molecular cloning a laboratory manual volume 1 pdf


molecular cloning a laboratory manual volume 1 pdf

3.26 5 Large-scale Preparation of Single-stranded and Double- stranded Bacteriophage M13 DNA.
; Molecular cloning - Laboratory manuals.
5.71 17 Pulsed-field Gel Electrophoresis via Transverse Alternating Field c c3 patch v1.09 Electrophoresis Gels.
5.47 11 Detection of DNA in Polyacrylamide Gels by Autoradiography.49 12 Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method.51 Introduction to Pulsed-field Gel Electrophoresis (Protocols 13-20).55 13 Preparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of DNA.Expanded scope: the nucleic-acid-based techniques selected for inclusion have promoted recent advances in gene transfer, protein expression, RNA analysis, and expression of cloned genes.6.28 7 Rapid Isolation of Yeast DNA.1.131 30 Screening Bacterial Colonies by Hybridization: Large Numbers.132 31 Lysing Colonies and Binding of DNA to Filters.2.61 14 Preparation of Bacteriophage DNA Cleaved with Two Restriction Enzymes for Use as a Cloning Vector.4.34 5 Working with Bacteriophage P1 and Its Cloning Systems.7.27 6 Separation of RNA According to Size: Electrophoresis of RNA through Agarose Gels Containing Formaldehyde.2.56 12 Extraction of Bacteriophage DNA from Large-scale Cultures Using Formamide.59 13 Preparation of Bacteriophage DNA Cleaved with a Single Restriction Enzyme for Use as a Cloning Vector.Essential appendices: an up-to-date collection of reagents, vectors, media, detection systems, and commonly used techniques are included.5.33 8 Alkaline Agarose Gel Electrophoresis.



(It) has once again established its primacy as the molecular laboratory manual and is likely to be found on lab ound delphi roady 2 users manual the world.
4.70 13 Analyzing Yeast Colonies by PCR.
1.119 27 Screening Bacterial Colonies Using X-gal and iptg: -Complementation.123 Alternative Protocol: Direct Application of X-gal and iptg to Agar Plates.125 28 Screening Bacterial Colonies by Hybridization: Small Numbers.126 29 "Screening Bacterial Colonies by Hybridization: Intermediate Numbers.129 Alternative Protocol: Rapid Lysis.
Plasmids and Their Usefulness in Molecular Cloning.
5.74 Alternative Protocol: Silver Staining pfge Gels.6.4 Additional Protocol: Estimating the Concentration of DNA by Fluorometry.12 2 Isolation of High-molecular-weight DNA from Mammalian Cells Using Formamide.13 3 Isolation of DNA from Mammalian Cells by Spooling.The addition of this information extends.Coli using Calcium Chloride.116 26 Transformation.2.87 21 Transfer of Bacteriophage DNA from Plaques to Filters.2.32 3 Preparing Stocks of Bacteriophage by Plate Lysis and Elution.34 Alternative Protocol: Preparing Stocks of Bacteriophage by Plate Lysis and Scraping.3.33 7 Analysis of Recombinant Bacteriophage M13 Clones.1.152 Purification of Plasmid DNA by PEG Precipitation.3.30 6 Cloning into Bacteriophage M13 Vectors.1.65 11 Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCI-Ethidium Bromide Gradients: Discontinuous Gradients.69 12 Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents.72 13 Removal of Ethidium Bromide from DNA by Ion-exchange Chromatography.75 14 Removal of Small.Users of the previous editions will recognize many of the organizational features in the experimental sections of this book.4.55 10 Working with Yeast Artificial Chromosomes.7.75 information panels How to Win the Battle with RNase.Nevertheless, the revision of the text has been extensive and detailed.



7.82 Inhibitors of RNases.83 Diethylpyrocarbonate.84 Guanidinium Salts.85 Nuclease.86 Exonuclease VII.86 Mung Bean Nuclease.87 Promoter Sequences Recognized by Bacteriophage-encoded RNA Polymerases.87 Actinomycin.88 index.1.88.

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